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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and so could lead to tumorigenesis. This protein was found to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't still obtainable.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.
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This guideline outlines more feasible get more info optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This manual outlines additional attainable optimization of cellular and immunoassay parameters to be certain the very best benefits are...
Antibodies certain to the blocking peptide not bind to your epitope about the target protein. By evaluating the staining within the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Info Cyclin E belongs for the extremely conserved cyclin household, whose associates are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation patterns which contribute into the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S period boundary which is degraded as cells progress through S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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